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ChIP (Chromatin Immunoprecipitation)
ChIP (Chromatin Immunoprecipitation) is a powerful molecular biology technique used to study protein-DNA interactions in cells. This article explores the principles, procedure, applications, and limitations of ChIP, providing insights into its significance in gene expression regulation and chromatin structure. Discover how ChIP has revolutionized research in genetics, epigenetics, and cancer research, while also exploring newer…
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DNA Footprinting
The DNA footprinting technique is used to study the interactions between DNA and proteins. This allows researchers to identify which regions of DNA are bound by specific proteins and how they interact with DNA. DNA footprinting has various methods like chemical, thermal, and electrophoretic mobility shift assays, which are used to map protein-DNA interactions, study…
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Electrophoretic Mobility Shift Assay: EMSA
The text describes Electrophoretic Mobility Shift Assay (EMSA), a molecular biology technique used to study protein-DNA interactions. EMSA is based on the principle that proteins can bind to specific regions of DNA and alter its conformation, affecting the mobility of the DNA in an electric field. This technique is used to study transcription regulation, DNA…
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