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Ion Exchange Chromatography
Introduction Ion exchange chromatography (IEC) is a type of liquid chromatography that separates molecules based on their charge. It is a widely used technique in biochemistry and analytical chemistry for the purification and isolation of biomolecules such as proteins, nucleic acids, and small molecules. Principles of Ion Exchange Chromatography IEC utilizes a stationary phase, which…
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Lac Operon- Gene Regulation in Bacteria
The lac operon, found in E. coli bacteria, is a remarkable genetic system that controls the metabolism of lactose, a sugar commonly present in milk. This operon comprises three crucial structural genes: lacZ, lacY, and lacA, responsible for encoding the essential enzymes needed to break down lactose into glucose and galactose. Its significance in the…
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Endocrine Organs of the Human Body
The endocrine system is a complex network of organs in the human body that produces and regulates hormones. These endocrine organs, including the pituitary gland, thyroid gland, adrenal glands, and others, play crucial roles in maintaining homeostasis, metabolism, reproduction, and overall health. Understanding the functions and interactions of these organs is essential for comprehending the…
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Beer-Lambert Law and Spectrophotometry
Discover the principles of the Beer-Lambert Law and its application in spectrophotometry. Explore how this law relates the concentration of a solute to absorbance, and its significance in quantitative analysis, chemical kinetics, environmental monitoring, pharmaceutical analysis, and biochemical assays. Unlock the power of spectrophotometry in understanding chemical substances and their characteristics.
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