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DNA Cloning
Introduction: Restriction Enzyme Cloning: PCR Cloning: Gene Synthesis: Conclusion:
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Restriction Enzymes
Introduction: Restriction Enzymes, also known as restriction endonucleases, are enzymes found in bacteria that play a crucial role in their defense mechanism. These enzymes recognize specific DNA sequences and cut the DNA at those sites, resulting in the restriction of foreign DNA from entering the bacterium. Restriction enzymes have become valuable tools in modern molecular…
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Restriction Mapping
Introduction Principles of Restriction Mapping Methods of Restriction Mapping Applications of Restriction Mapping Conclusion
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Gene Therapy: A study note
Gene therapy is a groundbreaking medical approach that holds tremendous potential for revolutionizing disease treatment. By altering the genetic material within cells, gene therapy aims to correct abnormal genes or introduce therapeutic genes to combat a wide range of conditions. This study note provides a comprehensive overview of gene therapy, exploring its principles, techniques, applications,…
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Restriction Endonucleases
Restriction endonucleases, the DNA-cutting enzymes, are indispensable in molecular biology research. Classified into four types based on recognition sites, Type II enzymes stand out as the most widely used. They cleave DNA at specific sequences, generating fragments with blunt or sticky ends. Despite their complexity, Type I and III enzymes have limited use due to…
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