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Ion Exchange Chromatography
Introduction Ion exchange chromatography (IEC) is a type of liquid chromatography that separates molecules based on their charge. It is a widely used technique in biochemistry and analytical chemistry for the purification and isolation of biomolecules such as proteins, nucleic acids, and small molecules. Principles of Ion Exchange Chromatography IEC utilizes a stationary phase, which…
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Circular Dichroism (CD) Technique
Circular dichroism (CD) is a powerful spectroscopic technique used to study the structure and properties of chiral molecules, such as proteins, nucleic acids, and carbohydrates. It is based on the differential absorption of left and right circularly polarized light by chiral molecules, which can provide information on their secondary, tertiary, and quaternary structures. In this…
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