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Multiphoton Laser Scanning Microscopy
Multiphoton microscopy utilizes the simultaneous absorption of two photons to excite fluorophores, enabling imaging with longer wavelength light. The process necessitates intense light sources, such as pulsed infrared lasers, to achieve sufficient photon density. Notably, this technique allows deeper tissue imaging, as biological specimens poorly absorb near-infrared radiation compared to UV and blue-green radiation. Additionally,…
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CLSM -Confocal Laser Scanning Microscopy
Confocal Laser Scanning Microscopy (CLSM) enables high-resolution imaging of biological specimens by focusing on thin layers within the sample. Utilizing lasers, pinhole apertures, and raster scanning, CLSM provides sharp, detailed images and facilitates three-dimensional reconstruction. This technique offers significant advantages in the precise localization and visualization of cellular structures, paving the way for advancements in…
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Objective Lenses of Microscopes
Discover the various types of objective lenses used in microscopes. Learn about achromatic, plan, apochromatic, and oil immersion lenses, their unique properties, and applications in microscopy. Choose the right lens for enhanced imaging and accurate observations.
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Confocal Microscope
Embark on a fascinating journey into the world of confocal microscopy. Discover its role in high-resolution imaging, sample preparation, and applications in biology, chemistry, and materials science. Uncover the groundbreaking advancements made possible by selectively detecting fluorescence, enabling detailed study of biological samples and components. Witness the transformative power of confocal microscopy in unlocking the…
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