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Isolation and Purification of RNA
Pure RNA isolation is essential in molecular biology for gene expression studies and downstream analyses. There are two main strategies: total RNA isolation and mRNA isolation, with total RNA isolation being less laborious. Methods include phenol-chloroform extraction, spin column purification, and magnetic bead-based methods, each having unique advantages. Choose based on sample specifics.
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Post transcriptional processingÂ
Post-transcriptional processing refers to the set of modifications and events that occur to the RNA molecule after transcription, but before translation. These modifications are important for the stability, localization, and translation of the RNA molecule. Capping, polyadenylation, splicing, RNA editing, RNA localization, and RNA stability are all important aspects of post-transcriptional processing.
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