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RFLP (Restriction Fragment Length Polymorphism)
The excerpt introduces RFLP, a molecular biology method for detecting DNA sequence variations. It explains the principle of restriction enzymes cutting specific DNA sequences and generating restriction fragments. The procedure involves DNA isolation, digestion, gel electrophoresis, and hybridization with labeled probes. RFLP’s applications span genetics, forensic science, and medical diagnostics. Despite its replacement by newer…
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Southern blotting
Southern blotting is a powerful technique used in molecular biology and genetics to analyze and identify specific DNA sequences. It involves the separation of DNA by electrophoresis and detection by hybridization. With applications in medical research, genetic engineering, diagnostics, and environmental monitoring, Southern blotting plays a crucial role in understanding DNA-related phenomena. Discover the principles,…
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