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Spectroscopy and Beer-Lambert’s Law
Spectroscopy, a fascinating scientific technique, delves into the captivating interaction between matter and electromagnetic radiation. By analyzing how substances interact with light at different wavelengths, spectroscopy provides crucial insights into the composition, structure, and behavior of materials. One of the fundamental principles in spectroscopy is the Beer-Lambert’s Law, a cornerstone in quantitative analysis. This law…
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Nuclear Magnetic Resonance (NMR)
The Nuclear Magnetic Resonance (NMR) technique is a powerful tool used in biophysics to study the structure, dynamics, and interactions of biomolecules in solution. NMR spectroscopy is based on the principle that certain atoms’ nuclei can absorb and emit electromagnetic radiation in the radiofrequency range when placed in a strong magnetic field. This technique can…
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Circular Dichroism (CD) Technique
Circular dichroism (CD) is a powerful spectroscopic technique used to study the structure and properties of chiral molecules, such as proteins, nucleic acids, and carbohydrates. It is based on the differential absorption of left and right circularly polarized light by chiral molecules, which can provide information on their secondary, tertiary, and quaternary structures. In this…
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